
HPLC Columns and Cartridges
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MilliporeSigma™ Hibar™ HPLC Columns, 250-4
Available in a variety of stationary phases that function in normal and/or reverse phase. MilliporeSigma Hibar™ HPLC Columns, 250-4 come with customized packing (please specify packing material) and are 250mmL x 4mmI.D.
Length (Metric) | 250 mm |
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Packing Material | Customized (specify packing material) |
Column Type | Hibar™ HPLC Column |
Diameter (Metric) | 4 mm |
Thermo Scientific™ ProPac™ IMAC-10 HPLC Columns
Resolve target proteins using a single column in a high-resolution gradient run with ProPac IMAC-10 LC Columns.
Pore Size | Nonporous |
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Particle Size | 10 μm |
Stationary Phase | Lysozyne Poly(IDA) Grafts |
Temperature | 60°C |
Phase | Affinity Separation |
Packing Material | Spherical, Nonporous Polymer |
Column Type | Separation of Metal-Binding Proteins |
pH | 2 to 12 |
Max. Pressure | 3000 psi (207 bar) |
Thermo Scientific™ ProSwift™ RP-2H PSDVB Reversed Phase HPLC Column, Monolith, 4.6 mm x 50 mm
Column Format: Analytical Column; Pore Size: Monolith; Column Phase: Reversed Phase
Thermo Scientific™ ProSwift™ SCX-1S Sulfonic Acid Ion Exchange HPLC Column, Monolith, 4.6 mm x 50 mm
Column Format: Analytical Column; Pore Size: Monolith; Column Phase: Ion Exchange
Thermo Scientific™ ProSwift™ RP-4H HPLC Columns

Run fast protein separations at high linear velocities with Thermo Scientific™ ProSwift™ RP-4H LC Columns.
Pore Size | Monolith |
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Solvents | Most common Organic Solvents |
Stationary Phase | Phenyl (Ph) |
Temperature | 70°C |
Phase | Reversed Phase |
For Use With | LC/MS |
Column Type | Reversed Phase |
Quantity | Each |
Green Features | Responsibly packaged |
Column Chemistry | Phenyl |
Column Format | Analytical Column |
Particle Size | Monolith |
Type | Analytical Column |
Packing Material | PSDVB (Polystyrene Divinylbenzene) |
Diameter (Metric) | 1 mm |
pH | 1 to 14 |
Technique | Bio-LC |
Product Line | Monolith |
Thermo Scientific™ Acclaim™ 120 C8 HPLC Columns
Achieve exceptional performance and maximum resolution for reversed-phase separation of analytes with moderate hydrophobicity.
Pore Size | 120 Å |
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Carbon Load | 11% |
Stationary Phase | C8 |
Temperature | 60°C |
Phase | Reversed Phase |
Endcapped | Yes |
Column Type | Reversed Phase |
pH | 2 to 8 |
Surface Area | 300 m²/g |
USP Type | L7 |
Thermo Scientific™ Acclaim™ Mixed Mode HILIC-1 HPLC Columns
Get all the advantages of reversed-phase and hydrophilic interaction liquid chromatography (HILIC) in one column with the Thermo Scientific™ Acclaim™ Mixed-Mode HILIC-1 column.
Pore Size | 120 Å |
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Stationary Phase | Hydrophobic Alkyl Chain with a Diol Group |
Temperature | 50°C |
Phase | Reversed Phase and HILIC |
Endcapped | Proprietary |
Column Type | Mixed Mode |
pH | 2.5 to 7.5 |
Green Features | Responsibly packaged |
Surface Area | 300 m²/g |
Product Line | Acclaim |
USP Type | N/A |
Thermo Scientific™ GlycanPac™ AXR-1 HPLC Columns
Get ultra-high resolution and selectivity for both labeled and native glycans using this HPLC/UHPLC column. It's compatible with MS and fluorescence detection.
Pore Size | 175 Å |
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Stationary Phase | Proprietary |
Temperature | 60°C |
Phase | Reversed Phase and Ion Exchange |
Column Type | Mixed Mode |
pH | 2 to 8 |
Product Line | GlycanPac |
Thermo Scientific™ Acclaim™ SEC-300 Size Exclusion Chromatography HPLC Columns
Separate water-soluble polymers and oligomers with the Acclaim family of resin-based, high-performance, size-exclusion chromatography columns.
Pore Size | 300 Å |
---|---|
Stationary Phase | Proprietary |
Temperature | 60°C |
Phase | Size Exclusion |
Endcapped | Yes |
Column Type | Size Exclusion |
Quantity | Each |
Green Features | Responsibly packaged |
Particle Size | 5 μm |
pH | 2 to 12 |
Technique | Bio-LC |
Particle Shape | Spherical |
Product Line | Acclaim |
USP Type | N/A |
Thermo Scientific™ HyPURITY™ C18 Columns, 5μm Particle Size
High quality, highly pure silica columns
Pore Size | 190 Å |
---|---|
Particle Size | 5 μm |
Carbon Load | 13% |
Stationary Phase | C18 |
Phase | Reversed Phase |
Endcapped | Yes |
Packing Material | Silica |
Column Type | Reversed Phase |
pH | 1 to 9 |
Surface Area | 200 m²/g |
USP Type | L1 |
Thermo Scientific™ HyPURITY™ ADVANCE™ HPLC Columns, 5μm Particle Size
Use this specialty C8 column for separating compounds showing poor peak shape with traditional alkyl bonded phases.
Particle Size | 5 μm |
---|---|
Carbon Load | 9% |
Stationary Phase | C8 |
Phase | Reversed Phase |
Packing Material | Silica |
Column Type | Reversed Phase |
Surface Area | 200 m²/g |
USP Type | L7 |
Thermo Scientific™ Hypersil™ Green PAH Columns, 3μm Particle Size
Specially tailored alkyl bonded silica with a high carbon loading, designed specifically for the analysis of polyaromatic hydrocarbons (PAHs)
Pore Size | 120 Å |
---|---|
Particle Size | 3 μm |
Temperature | 60°C |
Phase | Reversed Phase |
Endcapped | Yes |
Column Type | PAH Analysis |
pH | 2 to 8 |
Surface Area | 170 m²/g |
Thermo Scientific™ Hypersil™ Green PAH Columns, 3 and 5μm Particle Size
Specially tailored alkyl bonded silica with a high carbon loading, designed specifically for the analysis of polyaromatic hydrocarbons (PAHs)
Pore Size | 120 Å |
---|---|
Particle Size | 5 μm |
Carbon Load | 13.5% |
Stationary Phase | Proprietary |
Temperature | 60°C |
Phase | Reversed Phase |
Endcapped | Yes |
Column Type | PAH Analysis |
pH | 2 to 9 |
Surface Area | 170 m²/g |
USP Type | L1 |
Thermo Scientific™ Hypersil™ Silica Normal Phase HPLC Column, 3 μm, 4.6 mm x 250 mm
Column Format: Analytical Column; Carbon Load: 0%; Pore Size: 120 Å; Column Phase: Normal Phase
Thermo Scientific™ Hypersil GOLD™ Amino HPLC Columns

Achieve outstanding peak shape and sensitivity in weak anion exchange, reversed phase and normal phase modes with Hypersil GOLD Amino LC Columns.
Pore Size | 175 Å |
---|---|
Carbon Load | 2% |
Stationary Phase | Amino (NH2) |
Temperature | 60°C |
Phase | Reversed Phase, Normal Phase, Ion Exchange, HILIC |
Endcapped | Yes |
Column Type | Mixed Mode |
pH | 2 to 8 |
Surface Area | 220 m²/g |
USP Type | L8 |